Normal Goat Serum from Cell Signaling Technology

Product Specs
ItemNormal Goat Serum
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5425S
Quantity10 ml
DiseaseNormal
TypeSerum
Research AreaRelated Products
ApplicationsIHC-P, IF-F, IF-IC
Source/Expression SystemGoat

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaquesNature biotechnologyMarch 1, 2024Hu X, White K, Olroyd AG, DeJesus R, Dominguez AA, Dowdle WE, Friera AM, Young C, Wells F, Chu EY, et al.control. Blocking was done with 1× Animal-Free Blocker (SP-5030-250, Thermo Fisher Scientific) in 2% NGS (5425S, Cell Signaling Technology) diluted in TBST (J77500.K2, Thermo Fisher Scientific). The primary antibody

(2) Mitochondrial-targeted antioxidant attenuates preeclampsia-like phenotypes induced by syncytiotrophoblast-specific Gαq signalingSci AdvDecember 1, 2023Megan A. Opichka, M. Christine Livergood, Kirthikaa Balapattabi, McKenzie L. Ritter, Daniel T. Brozoski, Kelsey K. Wackman, Ko-Ting Lu, Kaleigh N. Kozak, Clive Wells, Agnes B. Fogo, et al.Samples were blocked with 3% hydrogen peroxide for 10 min and 5% normal goat serum (Cell Signaling, 5425) in tris-buffered saline with Tween 20 (Cell Signaling, 9997) for 1 hour. CD31 rabbit monoclonal antibody

(3) The BRD4-NUT fusion alone drives malignant transformation of NUT carcinomaCancer ResearchDecember 1, 2023R. Taylor Durall, Julianna Huang, Luke Wojenski, Yeying Huang, Prafulla C. Gokhale, Brittaney A. Leeper, Joshua O. Nash, Pedro L. Ballester, Scott Davidson, Adam Shlien, et al.and permeabilized using 0.3% Triton X-100. The cells were then blocked in 5% normal goat serum (CST, 5425) prepared in PBS + 0.3% Triton X-100. The cells were incubated with primary antibody dissolved in 1%

(4) Targeting wild-type IDH1 enhances chemosensitivity in pancreatic cancerbioRxivNovember 15, 2023Mehrdad Zarei, Omid Hajihassani, Jonathan J. Hue, Hallie J. Graor, Luke D. Rothermel, Jordan M. Wintersections were then incubated with a blocking solution containing 5% goat serum (Cell Signaling Technology; 5425) at room temperature for 1 hour. The primary antibodies were diluted (SignalStain Antibody Diluent; 8112

(5) Hippocampal injections of soluble amyloid-beta oligomers alter electroencephalographic activity during wake and slow-wave sleep in ratsAlzheimer's Research & TherapyOctober 13, 2023Audrey Hector, Chloé Provost, Benoît Delignat-Lavaud, Khadija Bouamira, Chahinez-Anissa Menaouar, Valérie Mongrain, Jonathan Brouillettefor 15 min in 10% formalin, washed with PBS, immersed in a blocking solution (PBS with 5% goat serum [5425; Cell Signaling Technology] and 0.3% Triton X-100 [BP151-500; Thermo Fisher Scientific]) for 60 min

(6) Three methods for examining the de novo proteome of microglia using BONCAT bioorthogonal labeling and FUNCAT click chemistrySTAR ProtocolsSeptember 1, 2023Alison Keolani Carlisle, Jürgen Götz, Liviu-Gabriel BodeaBovine Serum Albumin (BSA),Bovogen,Cat # BSAS 0.1 Normal goat serum (NGS),Cell Signaling Technology,Cat # 5425S Penicillin / Streptomycin,Gibco,Cat # 15140-122 "0.25% Trypsin-EDTA (1×), 100 mL",Gibco,Cat # 25200-056

(7) BRAF(V600E) mutation together with loss of Trp53 or pTEN drives the origination of hairy cell leukemia from B-lymphocytesMolecular cancerAugust 5, 2023Yap J, Yuan J, Ng WH, Chen GB, Sim YRM, Goh KC, Teo J, Lim TYH, Goay SM, Teo JHJ, et al.(#S1699, Dako) or citrate (pH 6.0) for 1 h. Next, all samples were blocked with normal goat serum (#5425, Cell Signaling Technologies) or bovine serum albumin for 1 h before incubating with primary antibody

(8) Generating a mouse model for relapsed Sonic Hedgehog medulloblastomaSTAR ProtocolsJune 1, 2023Allie Heller, Fang Du, Yongqiang Liu, Yijun Yang, Zeng-Jie YangDPBS, calcium, magnesium",Thermo Fisher,Cat# 14040133 Normal goat serum (NGS),Cell Signaling,Cat# 5425S Saline solution,Thermo Fisher,Cat# R064432 Ketamine,Vedco,Cat# AHO2WWH Xylazine,Bimdea-MTC,Cat# 1XYL0038XYL006 D-Mannitol,Sigma-Aldrich,Cat#

(9) Alterations in the preneoplastic breast microenvironment of BRCA1/2 mutation carriers revealed by spatial transcriptomicsbioRxiv : the preprint server for biologyMay 25, 2023Caputo A, Vipparthi K, Bazeley P, Downs-Kelly E, McIntire P, Ni Y, Hu B, Keri RA, Karaayvaz M.that, cells were washed twice with PBS containing 10% normal goat serum (Cell Signaling Technology, 5425S) before being permeabilized for 5 minutes with PBS containing 0.1% Triton X-100 (Sigma Aldrich, T8787–50ML).

(10) Wnt Signaling Activation in Gingival Epithelial Cells and Macrophages of Experimental PeriodontitisDentistry JournalMay 9, 2023Ying Chen, Yang Hublocking with 1% BSA (Cell Signaling, Danvers, MA, USA, #9998) and 10% goat serum (Cell Signaling, #5425). Primary antibodies used in the study were rabbit anti-TNFα antibody (Abcam, Cambridge, UK, ab 6671,

(11) Electrotaxis of alveolar epithelial cells in direct-current electric fieldsChinese Journal of TraumatologyMay 1, 2023Chao-Yue Yang, Jian-Hui Sun, Kan Zhu, Juan Du, Ying Zhang, Cong-Hua Lu, Wen-Yi Liu, Ke-Jun Zhang, An-Qiang Zhang, Ling Zeng, et al.for nonspecific binding was performed utilizing 10% normal goat serum (Cell Signaling Technology, #5425S, Massachusetts, USA) for BEAS-2B cells and 10% donkey serum (Abcam, ab7475, Cambridge, England) for

(12) Metformin regulates expression of DNA methyltransferases through the miR-148/-152 family in non-small lung cancer cellsClinical EpigeneticsMarch 23, 2023Bo Bin Lee, Dongho Kim, Yujin Kim, Joungho Han, Young Mog Shim, Duk-Hwan Kimincubated with primary antibodies specific for DNMT1, DNMT3a, and DNMT3b (Cell Signaling Technology 5425, Danvers, MA) at 4 °C overnight. These membranes were then washed with TBS-T three times (5 min each)

(13) Comprehensive Analyses of Muscle Function, Lean and Muscle Mass, and Myofiber Typing in MiceBIO-PROTOCOLFebruary 20, 2023Hima Bindu Durumutla, Chiara Villa, Manoj Panta, Michelle Wintzinger, Ashok Daniel Prabakaran Pragasam, Karen Miz, Mattia Quattrocelli)Triton X-100 (Sigma-Aldrich, catalog number: T8787)10% normal goat serum (Cell Signaling, catalog number: 5425)Ethanol, 200 proof (Thermo Scientific, catalog number: T038181000)Isoflurane (Covetrus, catalog number:

(14) Autolysosomal activation combined with lysosomal destabilization efficiently targets myeloid leukemia cells for cell deathFrontiers in OncologyFebruary 1, 2023Harshit Shah, Metodi Stankov, Diana Panayotova-Dimitrova, Amir Yazdi, Ramachandramouli Budida, Jan-Henning Klusmann, Georg M. N. Behrenswere incubated in antibody dilution buffer containing 5% goat serum (Cell Signaling Technology, No. 5425), 0.1% Triton X-100 (Sigma-Aldrich, 93443), and respective antibody at its respective dilution for 60

(15) Finerenone, a Non-Steroidal Mineralocorticoid Receptor Antagonist, Reduces Vascular Injury and Increases Regulatory T-Cells: Studies in Rodents with Diabetic and Neovascular RetinopathyInternational journal of molecular sciencesJanuary 25, 2023Jerome JR, Deliyanti D, Suphapimol V, Kolkhof P, Wilkinson-Berka JL.[67]. Three-micrometer paraffin sections were incubated overnight at 4°C with 10% normal goat serum (5425S, Cell Signaling Technology, Massachusetts, USA) followed by overnight incubation at 4°C with a rabbit

(16) BTG1 mutation yields supercompetitive B cells primed for malignant transformationScienceJanuary 20, 2023Coraline Mlynarczyk, Matt Teater, Juhee Pae, Christopher R. Chin, Ling Wang, Theinmozhi Arulraj, Darko Barisic, Antonin Papin, Kenneth B. Hoehn, Ekaterina Kots, et al.4°C. Fixed and permeabilized cells were blocked with 2% normal goat serum (Cell Signaling Technology 5425) diluted in perm buffer, for 15 min at room temperature. We then stained cells with AF647 anti-MYC (rabbit,

(17) Liquid Biopsy Detecting Circulating Tumor Cells in Patients with Non-Small Cell Lung Cancer: Preliminary Results of a Pilot StudyBiomedicinesJanuary 7, 2023Maria Giovanna Mastromarino, Sara Parini, Danila Azzolina, Sara Habib, Marzia Luigia De Marni, Chiara Luise, Silvia Restelli, Guido Baietto, Elena Trisolini, Fabio Massera, et al.follows: after resting for 1 h with 5% Blocking Buffer Normal Goat Serum (NGS, Cell Signaling, USA-5425S), the slides were incubated with the primary antibodies, anti-mouse PK (Cell Signaling, USA-4545S) to

(18) A transcription factor atlas of directed differentiationCellJanuary 5, 2023Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, et al.Pierce ECL Western Blotting Substrate,Thermo Fisher Scientific,32209 paraformaldehyde,VWR,15710 goat serum,Cell Signaling Technologies,5425S Triton X-100,MilliporeSigma,93443 ProLong Gold Antifade Mountant with DAPI,Thermo Fisher Scientific,P36941 Formaldehyde,MilliporeSigma,252549 glycine,MilliporeSigma,G7126 Protein

(19) Brain microvascular endothelial cell dysfunction in an isogenic juvenile iPSC model of Huntington’s diseaseFluids and Barriers of the CNSDecember 1, 2022Raleigh M. Linville, Renée F. Nerenberg, Gabrielle Grifno, Diego Arevalo, Zhaobin Guo, Peter C. Searsonfixed with ice cold methanol for 15 min, and blocked with 10% goat serum (Cell Signaling Technology #5425) or 10% donkey serum (Millipore Sigma #D9663) supplemented with 0.3% Triton X-100 (Millipore Sigma #108643)

(20) Reciprocal effects of mTOR inhibitors on pro-survival proteins dictate therapeutic responses in tuberous sclerosis complexiScienceNovember 18, 2022McNamara MC, Hosios AM, Torrence ME, Zhao T, Fraser C, Wilkinson M, Kwiatkowski DJ, Henske EP, Wu CL, Sarosiek KA, et al.temperature in tris-buffered saline with 0.1% Tween 20 (TBST) containing 5% normal goat serum (CST, #5425). Sections were then incubated overnight at 4°C with the following primary antibodies diluted in SignalStain Antibody Diluent

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Normal Goat Serum from Cell Signaling Technology

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Normal Goat Serum from Cell Signaling Technology

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